博士、讲师

简历：

主要科研领域与方向:

1.       杆状病毒分子生物学研究，解析杆状病毒基因功能和家蚕抗病毒机制研究，阐释病毒劫持宿主能量代谢、病毒阻滞细胞周期进程和家蚕抗病毒免疫等病原和宿主相互博弈机制；

2.       家蚕新型基因编辑系统创制与应用研究，培育家蚕抗病、蚕丝和抗逆等基因编辑创新素材，以及家蚕转基因实用品种遗传改良和推广应用研究。

科学成就：

先后主持包括国家自然科学基金、中国博士后科学基金、重庆市自然科学基金等项目7项，作为骨干人员参与国家蚕桑产业技术体系、国家“863”计划项目和国家自然科学基金等项目。以第一作者在国内外重要学术期刊Antiviral Research、Frontiers in Microbiology、Applied Microbiology and Biotechnology和Journal of Biological Engineering等发表20余篇SCI等论文，申请专利6项，担任Frontiers in Microbiology和Cell Cycle等杂志审稿人。获得重庆市自然科学二等奖（排名第4）、重庆市优秀博士论文和重庆市优秀博士毕业研究生等荣誉。

承担科技项目：

1、国家自然科学基金青年项目：家蚕ATPase家族ATAD3A基因调控宿主能量代谢机制研究（31902214）（主持）；

2、中国博士后科学基金面上项目：基于CRISPR/Cas9的家蚕抗BmNPV遗传改良研究（No. 2018M633309）（主持）；

3、重庆市博士后特别资助项目：家蚕新型抗性素材创制研究（XmT2018020）（主持）；

4、重庆市自然科学基金项目：人工合成杆状病毒诱导型启动子P39K的构建及应用（cstc2019jcyj-msxm X0096）（主持）；

5、国家自然科学基金地区基金合作项目：家蚕病原性微孢子虫感染特性及其胚种传染机理研究（32060777）（合作研究）；

6、中央高校基本科研业务费：人工合成病毒诱导型启动子P39K的构建及应用研究（XDJK2020C010）（主持）；

7、西南大学博士基金项目：家蚕核型多角体病毒IE-2对宿主细胞周期的调控机制研究（SWU120008）（主持）；

8、中央高校基本科研业务费：家蚕核型多角体病毒LEF-11与宿主相互作用蛋白的鉴定（XDJK2015D002）（主持）；

9、国家蚕桑产业技术体系：家蚕微粒子检疫检测（NO. CARS-18）（主要成员）；

10、国家自然科学基金面上项目：家蚕核型多角体病毒lef-11基因对病毒增殖的调控研究（NO. 31472152）（主要完成人）；

11、国家自然科学基金面上项目：家蚕核型多角体病毒调控细胞周期机制研究（NO. 31872427）（主要参与者）；

12、国家自然科学基金青年项目：凋亡抑制基因在家蚕和BmNPV互作中的功能研究（NO. 31602009）（主要参与者）。

代表性论文/论著：

[1] Dong ZQ, Qin qi, Hu Z, Zhang Xinling, Miao Jianghao, Huang L, Chen P, Lu C*, Pan MH*CRISPR/Cas12a Mediated Genome Editing Enhances Bombyx mori Resistance to BmNPV. Front Bioeng Biotechnol. 2020 Jul 15;8:841.

[2] Dong ZQ, Long JQ, Huang L, Hu Z, Chen P, Hu N, Zheng N, Huang XH, Lu C*, Pan MH*. Construction and application of an HSP70 promoter-inducible genome editing system in transgenic silkworm to induce resistance to Nosema bombycis. Applied Microbiology and Biotechnology. 2019 Dec;103(23-24):9583-9592.

[3] Dong ZQ, Qin Q, Hu Z, Chen P, Huang L, Zhang X, Tian T, Lu C*, Pan MH*. (2014) Construction of a one-vector multiplex CRISPR/Cas9 editing system to inhibit nucleopolyhedrovirus replication in silkworms. Virologica Sinica. 2019 Aug;34(4):444-453.

[4] Dong ZQ, Hu ZG, Li HQ, Jiang YM, Cao MY, Chen P, Lu C*, Pan MH*. Construction and characterization of a synthetic Baculovirus-inducible 39K promoter. Journal of Biological Engineering, 2018 Dec 4;12:30.

[5] Dong ZQ, Huang L, Dong FF, Hu ZG, Qin Q, Long JQ, Chen P, Lu C*, Pan MH*. Establishment of a baculovirus-inducible CRISPR/Cas9 system for antiviral research in transgenic silkworms. Applied Microbiology and Biotechnology. 2018,102: 9255.

[6] Dong ZQ, Hu ZG, Qin Q, Dong FF, Huang L, Long JQ, Chen P, Lu C*, Pan MH*.  CRISPR/Cas9 mediated disruption of the ie-0 and ie-2 as a therapeutic approach to BmNPV in transgenic silkworm. Insect Molecular Biology. 2018 Aug 18. doi: 10.1111/imb.12529

[7] Dong ZQ, Dong FF, Yu XB, Huang L, Jiang YM, Hu ZG, Chen P, Lu C*, Pan MH*. Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System. Frontiers in Microbiology. 2018 Feb 16;9:209.

[8] Dong ZQ, Hu N, Dong FF, Chen TT, Jiang YM, Chen P, Lu C*, Pan MH*. Baculovirus LEF-11 Hijack Host ATPase ATAD3A to Promote Virus Multiplication in Bombyx mori cells. Scientific Reports. 2017 Apr 10; 7:46187. 

[9] Dong ZQ, Chen TT, Zhang J, Hu N, Cao MY, Dong FF, Jiang YM, Chen P, Lu C*, Pan MH*. Establishment of a highly efficient virus-inducible CRISPR/Cas9 system in insect cells. Antiviral Research. 2016,130:50-57.

[10] Dong ZQ, Hu N, Zhang J, Chen TT, Cao MY, Li HQ, Lei XJ, Chen P, Lu C*, Pan MH*. (2015) Oligomerization of Baculovirus LEF-11 Is Involved in Viral DNA Replication. PLoS One. 2015 Dec 14;10(12):e0144930.

[11]  Dong ZQ, Zhang J, Chen XM, He Q, Cao MY, Wang L, Li HQ, Dong XL, Pan CX, Lu C*, Pan MH*. Bombyx mori nucleopolyhedrovirus ORF79 is a per os infectivity factor associated with the PIFs complex. Virus Research. 2014 Feb 26.184:62–70.

[12]  Chen TTδ, Dong ZQδ, Hu N, Hu ZG, Dong FF, Jiang YM, Li J, Chen P, Lu C, Pan MH*. Baculovirus LEF-11 nuclear localization signal is important for viral DNA replication. Virus Research. 2017 Jun 22, pii: S0168-1702(17)30334-9.(Co-first author)

[13]  Hu ZG#, Dong ZQ#, Dong FF, Zhu Y, Chen P, Lu C*, Pan MH*. Identification of a PP2A gene in Bombyx mori with antiviral function against B. mori nucleopolyhedrovirus, Insect Science, 2019 May 9. doi: 10.1111/1744-7917.12678. (Co-first author)

[14]  Zhang J, Dong ZQ, He Q, Chen XM, Cao MY, Li HQ, Xiao WF, Lu C*, Pan MH*. Identification of a novel nuclear localization signal of baculovirus late expression factor 11. Virus Research.2014 May 12,184:111–119.

[15] Huang Lδ, Dong ZQδ, Dong FF, Yu XB, Hu ZG, Liao NC, Chen P, Lu C, Pan MH. Gene editing the BmNPV inhibitor of apoptosis protein 2 (iap2) as an antiviral strategy in transgenic silkworm. Int J Biol Macromol. 2020 Oct 29:S0141-8130(20)34863-7. (Co-first author)

[16] Hu Nδ, Dong ZQδ, Chen TT, Zheng N, Wu Q, Chen P, Lu C, Pan MH. A novel system to rapidly detect protein-protein interactions (PPIs) based on fluorescence co-localization. Biotechnol Lett. 2020 Nov;42(11):2111-2122. (Co-first author)

[17] Chen P, Kang TT, Bao XY, Dong ZQ, Zhu Y, Xiao WF, Pan MH, Lu C*，Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs. Insect Science, 2019 Jan 30. doi: 10.1111/1744-7917.12664.

[18]  Chen TT, Tan LR, Hu N, Dong ZQ, Jiang YM, Hu ZG, Chen P, Pan MH*, Lu C*. C-lysozyme contributes to antiviral immunity in Bombyx mori against nucleopolyhedrovirus infection. Journal of Insect Physiology. 2018.05.005.

[19]  Chen TT, Tan LR, Hu N, Dong ZQ, Hu ZG, Qin Q, Long JQ, Chen P, Xu AY, Pan MH, Lu C. Specific genes related to nucleopolyhedrovirus in Bombyx mori susceptible and near-isogenic resistant strains through comparative transcriptome analysis, Insect Mol Biol. 2019 Jan 10. doi: 10.1111/imb.12566.

[20]  Wang L, Xiao Q, Zhou XL, Zhu Y, Dong ZQ, Chen P, Pan MH*, Lu C*. Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) Induces Host Cell Autophagy to Benefit Infection. Viruses. 2017. 10(1):14.

[21]  Xiao Q, Wang, Zhou XL, Zhu Y, Dong ZQ, Chen P, Lu C*, Pan MH*. BmAtg13 promotes the replication and proliferation of Bombyx mori nucleopolyhedrovirus. Pestic Biochem Physiol. 2019 Jun;157:143-151.

[22]  Zhang J, Chen XM, Zhang CD, He Q, Dong ZQ, Cao MY, Dong XL, Pan CX, Lu C*, Pan MH*. Differential susceptibilities to BmNPV infection of two cell lines derived from a single silkworm ovarian tissue. PLoS One. 2014 September 15. 9(9) , e105986.

[23]  Zhang J, He Q, Zhang CD, Chen XY, Chen XM, Dong ZQ, Li N, Kuang XX, Cao MY, Lu C*, Pan MH*. Inhibition of BmNPV replication in silkworm cells using inducible and regulated artificial microRNA precursors targeting the essential viral gene lef-11. Antiviral Research. 2014 Jan 31.104 :143–152.

[24]  Cao MY, Kuang XX, Li HQ, Lei XJ, Xiao WF, Dong ZQ, Zhang J, Hu N, Chen TT, Lu C*, Pan MH*. Screening and optimization of an efficient Bombyx mori nucleopolyhedrovirus inducible promote. Journal of Biotechnology. 2016 May 28. [Epub ahead of print]

[25] 董战旗，蒋亚明，潘敏慧，家蚕热休克蛋白HSP60相互作用蛋白筛选与鉴定，中国农业科学 2019 第52卷 第2期 P376-384  0578-1752。

[26] 董战旗，蒋亚明，陈鹏，潘敏慧，家蚕ADP/ATP转运酶（BmANT）抑制BmNPV增殖作用机制研究，微生物学报，2019, 59(8): 1474–1483。

[27] 董战旗，雷雪蛟，秦琪，张新铃，唐亮，石美宁，潘敏慧，家蚕抗性品系NC99R抗BmNPV作用机制分析，生物工程学报，2019 1000-3061。

[28] 董战旗，潘敏慧*，对家蚕抗核型多角体病毒的研究策略及研究进展，蚕业科学，0139-0146（2017）01-0139-08.

[29] 董战旗，胡楠，陈婷婷，董非凡，蒋亚明，鲁成，潘敏慧*，家蚕核型多角体病毒(BmNPV) lef-11基因在病毒感染宿主细胞中的表达特征，蚕业科学，0257-4799（2016）02-07.

[30] 董战旗，张军，胡楠，鲁成，潘敏慧*，家蚕核型多角体病毒IE1的多克隆抗体制备及鉴定.西南大学学报，1673-9868（2014）10-0076-06.

联系地址：重庆市北碚区西南大学（南区）家蚕基因组生物学国家重点实验室，400716

电    话/微信；18883252030；QQ:691803860；E-mail：zqdong@swu.edu.cn；

个人主页：https://www.researchgate.net/profile/Zhanqi_Dong；https://orcid.org/0000-0002-0607-4241；