潘敏慧
发布时间:2016-11-25 查看 0次   字体:[] [] []


教授、博士生导师

国家蚕桑产业技术体系岗位科学家


简历:

2007-       西南大学          教授

2002-2007     西南大学          副教授

1997-2002     西南农业大学        讲师

1997-1998     日本信州大学纤维学部    留学

2002-2005     西南农业大学        博士研究生

1997-2001     西南农业大学        硕士研究生

1988-1992     西南农业大学        本科生


主要科研领域与方向:

家蚕细胞凋亡与周期调控、家蚕病理学与蚕病防控


科学成就:

在家蚕病原微生物分类、病理生物学和细胞生物学等领域取得了较好的研究业绩。发现和鉴定了一种新的家蚕病原微孢子虫SCM8和一种新型家蚕浓核病毒BmDNV-6;构建了家蚕细胞生物学研究平台,建立了多个家蚕胚胎细胞系和家蚕卵巢细胞系;在家蚕细胞凋亡和蚕病综合防控方面取得了重要研究成果。先后主持国家自然科学基金、国家支撑计划和省部级项目8项,主研“863”、“973”、“国家攻关计划”和省部级项目17项。在国内外重要学术杂志上发表研究论文130余篇,获得授权发明专利4项,参与出版专著4部。


代表性论文/论著:

[1] Dong ZQ, Huang L, Dong FF, Hu ZG, Qin Q, Long JQ, Cao MY, Chen P, Lu C*, Pan MH  Establishment of a baculovirus-inducible CRISPR/Cas9 system for antiviral research in transgenic silkworms. Appl Microbiol Biotechnol. 2018 Aug 28.

[2] Dong ZQ, Hu ZG, Qin Q, Dong FF, Huang L, Long JQ, Chen P, Lu C*, Pan MH* CRISPR/Cas9 Mediated Disruption of the ie-0 and ie-2 as a Therapeutic Approach to BmNPV in Transgenic Silkworm. Insect Mol Biol. 2018 Aug 18.

[3] Tang XF, Zhou XL, Zhang Q, Chen P, Lu C*, Pan MH*. Bombyx mori cyclin-dependent kinase inhibitor is involved in regulation of the silkworm cell cycle. Insect Mol Biol. 2018 Jun;27(3):404-413.

[4] Dong ZQ, Dong FF, Yu XB, Huang L, Jiang YM, Hu ZG, Chen P, Lu C*, Pan MH*. Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System. Front Microbiol. 2018 Feb 16;9:209.

[5] Yang JG, Liu TH, Dong XL, Wu YF, Zhang Q, Zhou L, Chen P, Lu C*, Pan MH*.In vivo RNA interference of BmNHR96 enhances the resistance of transgenic silkworm to BmNPV. Biochem Biophys Res Commun. 2017 Nov 4;493(1):332-339.

[6] Chen TT, Dong ZQ, Hu N, Hu ZG, Dong FF, Jiang YM, Li J, Chen P, Lu C*, Pan MH*. Baculovirus LEF-11 nuclear localization signal is important for viral DNA replication. Virus Res. 2017 Jun 15;238:133-140.

[7] Dong ZQ, Hu N, Dong FF, Chen TT, Jiang YM, Chen 1, Lu C*, Pan MH*. Baculovirus LEF-11 Hijack Host ATPase ATAD3A to Promote Virus Multiplication in Bombyx mori cells. Sci Rep. 2017 Apr 10;7:46187.

[8] Tang XF, Chen XY, Zhang CD, Li YF, Liu TH, Zhou XL, Wang L, Zhang Q, Chen P, Lu C*, Pan MH*. Two Geminin homologs regulate DNA replication in silkworm, Bombyx mori. Cell Cycle. 2017 May 3;16(9):830-840.

[9] Cao MY, Kuang XX, Li HQ, Lei XJ, Xiao WF, Dong ZQ, Zhang J, Hu N, Chen TT, Lu C*, Pan MH*. Screening and optimization of an efficient Bombyx mori nucleopolyhedrovirus inducible promote. Journal of Biotechnology.2016 Aug 10;231:72-80.

[10] Liu TH, Dong XL, Pan CX, Du GY, Wu YF, Yang JG, Chen P, Lu C*, Pan MH*. A newly discovered member of the Atlastin family, BmAtlastin-n, has an antiviral effect against BmNPV in Bombyx mori. Sci Rep. 2016 Jun 29;6:28946.

[11] Dong ZQ, Chen TT, Zhang J, Hu N, Cao MY, Dong FF, Jiang YM, Chen P, Lu C*, Pan MH*. Establishment of a highly efficient virus-inducible CRISPR/Cas9 system in insect cells. Antiviral Res. 2016 Jun;130:50-7.

[12] Dong XL, Liu TH, Wang W, Pan CX, Wu YF, Du GY, Chen P, Lu C*, Pan MH*.BmREEPa Is a Novel Gene that Facilitates BmNPV Entry into Silkworm Cells. PLoS One. 2015 Dec 14;10(12):e0144575.

[13] Dong ZQ, Hu N, Zhang J, Chen TT, Cao MY, Li HQ, Lei XJ, Chen P, Lu C*, Pan MH*. Oligomerization of Baculovirus LEF-11 Is Involved in Viral DNA Replication. PLoS One. 2015 Dec 14;10(12):e0144930.

[14] Feng ZY,Pan C,Liu M,Tian ZQ,Chen XM,Kuang XX,Lu C Pan MH*. Establishment and Gene Expression Characteristics of the Silk Gland Cell Line BmSG-SWU1 of Bombyx mori (Lepidoptera: Bombycidae). Ann. Entomol. Soc. Am.2014 Sep,107(99):1018-1026.

[15] Zhang J, Chen XM, Zhang CD, He Q, Dong ZQ, Cao MY, Dong XL, Pan CX, Lu C*, Pan MH*. Differential susceptibilities to BmNPV infection of two cell lines derived from a single silkworm ovarian tissue. PLoS One.2014 September 15. 9(9) , e105986.

[16] Zhang J, Dong ZQ, He Q,Chen XM, Cao MY, Li HQ, Xiao WF,Lu C*, Pan MH*  Identification of a novel nuclear localization signal of baculovirus late expression factor 11. Virus Res.2014  May 12,184:111–119.

[17] Dong ZQ, Zhang J, Chen XM,He Q, Cao MY,Wang L,Li HQ, Dong XL, Pan CX, Lu C*,Pan MH*. Bombyx mori nucleopolyhedrovirus ORF79 is a per os infectivity factor ssociated with the PIFs complex. Virus Res.2014 Feb 26.184:62–70.

[18] Zhang J, He Q, Zhang CD, Chen XY, Chen XM, Dong ZQ, Li N, Kuang XX, Cao MY, Lu C*, Pan MH*. Inhibition of BmNPV replication in silkworm cells using inducible and regulated artificial microRNA precursors targeting the essential viral gene lef-11. Antiviral Res. 2014 Jan 31.104 :143–152.

[19] Pan MH, Hong KL, Chen XY, Pan C, Chen XM, Kuang XX, Lu C. BmCyclin B3 are required for cell cycle progression of silkworm, Bombyx mori. Sci China Life Sci. 2013 Apr;56(4):360-5.

[20] Pan MH, Du J, Zhang JY, Huang MH, Li T, Cui HJ, Lu C*. Cloning of the Flap Endonuclease-1 Gene in Bombyx mori and Identification of an Antiapoptotic Function. DNA and Cell Biology. 2011 Oct;30(10):763-70.

[21] Zhang JY*, Pan MH*, Sun ZY, Huang SJ, Yu ZS, Liu D, Zhao DH, Lu C. The genomic underpinnings of apoptosis in the silkworm, Bombyx mori. BMC Genomics. 2010;11(1):611. (*, co-first author).

[22] Pan MH, Cai XJ, Liu M, Lv J, Tang H, Tan J, Lu C. Establishment and Characterization of an Ovarian Cell Line of the Silkworm, Bombyx mori. Tissue Cell ,2010, 42:42-46.

[23] Pan MH, Wang XY, Chai CL, Zhang CD, Lu C*, Xiang ZH. Identification and function of Abdominal-A in the Silkworm, Bombyx mori.  Insect Molecular Biology,2009,18(2):155–160

[24] Pan MH, Yu QY, Xia YL, Dai FY, Liu YQ,Lu C*, Zhang Z,Xiang ZH. Characterization of the mitochondrial genome of the Chinese wild mulberry silkworm, Bombyx mandarina (Lepidoptera: Bombycidae). Sci China ser C-Life Sci,2008,51(8):693-701.

[25] Pan MH, Xiao SQ, Chen M, Hong XJ, Lu C* . Establishment and characterization of two enbryonic cell lines of Bombyx mori. In Vitro Cell.Dev.Biol-Animal, 2007, 43:101-104.

*,corresponding author


联系地址:重庆市北碚区西南大学(南区)家蚕基因组生物学国家重点实验室,400716

电   话: 023-68250076

E-mail :pmh047@126.com





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