| 发布时间：2020-12-10 查看 0次 字体：[大] [中] [小] |
副教授
国际学术身份认证编码(ORCID)：https://orcid.org/0000-0002-2907-6901.
学习与工作经历
2006.09-2010.06 西南大学生物技术学院 生物技术专业 理学学士
2010.09-2012.06 西南大学家蚕基因组生物学国家重点实验室 生物工程专业 工学硕士
2012.09-2015.06 西南大学家蚕基因组生物学国家重点实验室 遗传学专业 理学博士
2015.07-2017.07 西南大学家蚕基因组生物学国家重点实验室 畜牧学专业 博士后
2017.08-2019.09 西南大学家蚕基因组生物学国家重点实验室 助理研究员
2018.09-2018.10 荷兰奈梅亨大学 访问学者
2019.10-2021.09 美国佐治亚州立大学 生物医学科学研究所 博士后研究助理
2020.06-今 西南大学家蚕基因组生物学国家重点实验室 副教授
主要科研领域与方向
（1） 家蚕安全转基因技术体系的建立及应用研究；
（2） 基于转基因技术和基因组编辑技术的新型蚕丝材料的创制及应用研究；
（3） 开发利用野生/转基因丝素蛋白作为载体的纳米药物，用于炎症性肠病（IBD）和直结肠癌（CRC）的治疗研究。
学术贡献
主要从事基于转基因技术和基因组编辑技术的家蚕遗传改良研究，基于蚕丝蛋白的纳米药物载体开发及炎症性肠病（IBD）和直结肠癌（CRC）的治疗研究。主持包括国家自然科学基金、中国博士后科学基金、重庆市基础科学与前沿技术研究专项、欧洲Erasmus+ Scholarship Project和美国Crohn’s and Colitis Foundation研究奖学金项目等多个国内或国际科研项目。在Matter、Bioactive Materials、Nanomedicine、FEBS J和Insect Biochemistry and Molecular Biology等学术期刊发表研究论文40余篇，其中30篇被SCI收录。申请发明专利8项，其中授权3项。作为主要撰稿人参与编写学术专著《转基因家蚕技术与应用》。受邀为Plant Biotechnology Journal、Acta Biomaterialia、Frontiers Cell and Developmental Biolog、Chemosphere等国际知名期刊担任审稿专家。受邀于美国、荷兰、古巴等国家举行的国际学术会议作英文报告。研究领域涉及桑树基因的功能和桑蚕的遗传改良研究，家蚕安全转基因技术体系建立，基于转基因和基因组编辑技术的蚕丝新材料的创制与研究，以及开发基于丝素和转基因丝蛋白的新型纳米药物载体，应用于炎症性肠病和结直肠癌的治疗研究。
发表学术论文
(1) Long D, Cheng X, Hao Z, Sun J, Umuhoza D, Liu Y, Chen L, Xiang Z, Yang F*, Zhao A*. “Genetic hybridization of highly active exogenous functional proteins into silk-based materials using ‘light-clothing’ strategy” Matter 2021, https://doi.org/10.1016/j.matt.2021.03.020.
(2) Cheng X, Long D, Chen L, Jansen J, Leeuwenburgh S, Yang F*. “Electrophoretic Deposition of Silk Fibroin Coatings with Pre-defined Architecture to Facilitate Precise Control Over Drug Delivery” Bioactive Materials 2021, 6: 4243-4254.
(3) Sung J, Wang L, Long D, Yang C*, Merlin D. “PepT1-Knockout Mice Harbor a Protective Metabolome Beneficial for Intestinal Wound Healing” AJP: Gastrointestinal and Liver Physiology 2021, https://doi.org/10.1152/ajpgi.00299.2020.
(4) Long D*, Yang C, Sung J, Merlin D. “Atomic Force Microscopy to Characteriz Ginger Lipid Derived Nanoparticles (GLDNP)” Bio-protocol 2021, 11(07): e3969.
(5) Hao Z, Long D, Zhang Y, Umuhoza D, Dai J, Xu Z, Zhang G. Meng W, Xiang Z, Zhao A*. “New insight into the mechanism of in vivo fibroin self-assembly and secretion in the silkworm, Bombyx mori” Int. J. Biol. Macromol. 2021, 169: 473-479.
(6) Chen Q, Ma Y, Bai P, Li Q, Canup B, Long D, Ke B, Dai F, Xiao B*, Li C. “Tumor microenvironment-responsive nanococktails for synergistic enhancement of cancer treatment via cascade reactions” ACS Appl. Mater. Interfaces 2021, 13: 4861-4873.
(7) Cao B, Xia Z, Hao Z, Liu C, Long D, Fan W, Zhao A*, “The C-terminal tail of the plant endosomal-type NHXs plays a key role in its function and stability” Plant Science, 2021, 303: 110791.
(8) Long D*, Xiao B, Merlin D. “Genetically modified silk fibroin nanoparticles for drug delivery: preparation strategies and application prospects” Nanomedicine 2020, 15(18): 1739-1742.
(9) Umuhoza D, Yang F, Long D, Hao Z, Dai J, Zhao A*. “Strategies for tuning the biodegradation of silk fibroin-based materials for tissue engineering applications” ACS Biomater. Sci. Eng. 2020, 6(3): 1290-1310.
(10) Liu R, Zeng W, Tan T, Chen T, Luo Q, Qu D, Tang Y, Long D, Xu H*. “Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system” Transgenic Res. 2019, 28(5-6):627-636.
(11) Liu C, Li J, Zhu P, Yu J, Hou J, Wang C, Long D, Yu M, Zhao A*. “Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression” Peer J, 2019, 7: e6391.
(12) Liu C, Xu Y, Feng Y, Long D, Cao B, Xiang Z, Zhao A*. “Ectopic expression of mulberry G-proteins alters drought and salt stress tolerance in tobacco” Int. J. Mol. Sci., 2019, 20(1): 89.
(13) Fan W, Liu C, Cao B, Qin M, Long D, Xiang Z, Zhao A*. “Genome-wide identification and characterization of four gene families putatively involved in cadmium uptake, translocation and sequestration in mulberry” Front. Plant Sci., 2018, 9: 879.
(14) Liu C, Xu Y, Fan W, Long D, Cao B, Xiang Z, Zhao A*. “Identification of the genes involved in heterotrimeric G-protein signaling in mulberry and their regulation by abiotic stresses and signal molecules” Biologia Plantarum, 2018, 62 (2): 277-286.
(15) Fan W, Guo Q, Liu C, Liu X, Zhang M, Long D, Xiang Z, Zhao A*. “Two mulberry phytochelatin synthase genes confer zinc/cadmium tolerance and accumulation in transgenic Arabidopsis and tobacco” Gene, 2018, 645: 95-104.
(16) Long D, Hao Z, Xiang Z, Zhao A*. “Progress in targeted genome editing technology in the silkworm, Bombyx mori” Acta Entomologica Sinica, 2018, 61(3): 371-384. (in Chinese)
(17) Long D, Hao Z, Liu D, Dai J, Xiang Z, Zhao A*. “Progress in establishment and application of conditional gene targeting technology in silkworm, Bombyx mori” Science of Sericulture, 2018, 44(1): 1-15. (in Chinese)
(18) Long D, Hao Z, Xiang Z, Zhao A*. “Current status of transgenic technologies for safety consideration in silkworm (Bombyx mori) and future perspectives” Scientia Agricultura Sinica, 2018, 51(2): 363-373. (in Chinese)
(19) Chen Y, Long D, Dou X, Qi R, Zhao A*. “Studies on the protein purification ability of an ELP30-Tag in prokaryotic expression system” China Biotechnology, 2018, 38(2):54-60. (in Chinese)
(20) Zhang K, Li C, Weng X, Su J, Shen L, Pan G, Long D, Zhao A, Cui H*. “Transgenic characterization of two silkworm tissue-specific promoters in the haemocyte plasmatocyte cells” Insect Mol Biol, 2018, 27(2): 133-142.
(21) Zhang Y, Zhao A, Long D, Bi L, Wei W, Lu C, Zhang G*. “A method for screening transgenic homozygote by mixed sperm fertilization of different marking varieties in Bombyx mori” Science of Sericulture, 2018, 44(5): 805-809. (in Chinese)
(22) Zhao A, Xiang Z, Long D, Xu H, Pan M, Hao Z, Li C, Xiang Z. “Transgenic silkworm techniques and applications” Shanghai Scientific and Technical Publishers, Dec. 2017. (in Chinese)
(23) Liu C, Xu Y, Long D, Cao B, Hou J, Xiang Z, Zhao A*. “Plant G-protein β subunits positively regulate drought tolerance by elevating detoxification of ROS” Biochem Biophys Res Commun, 2017, 491: 897-902.
(24) Liu C, Liu X, Long D, Cao B, Xiang Z, Zhao A*. “De novo assembly of mulberry (Morus alba L.) transcriptome and identification of candidate unigenes related to salt stress responses” Russian Journal of Plant Physiology, 2017, 64(5): 738-748.
(25) Long D, Lu W, Hao Z, Xiang Z, Zhao A*. “Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system” Transgenic Res, 2016, 25(6):795-811.
(26) Cao B#, Long D#, Zhang M, Liu C, Xiang Z, Zhao A*. “Molecular characterization and expression analysis of the mulberry Na+/H+ exchanger gene family” Plant Physiol Biochem, 2016, 99: 49-58. (# These authors contributed equally to this paper)
(27) Long D, Lu W, Zhang Y, Guo Q, Xiang Z, Zhao A*. “New insight into the mechanism underlying fibroin secretion in silkworm, Bombyx mori” FEBS J., 2015, 282(1): 89-101.
(28) Long D, Lu W, Zhang Y, Bi L, Xiang Z, Zhao A*. “An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori” Scientific Reports, 2015, 5: 8802.
(29) Bian C, Long D, Liu X, Wei C, Gong J, Zhao A*. “Cloning and expression to salt stress of Na+/H+ antiporter gene (MnNHX1) in mulberry tree” Scientia Silvae Sinicae, 2015, 51(8): 16-25. (in Chinese)
(30) Wei C, Liu X, Long D, Guo Q, Fang Y, Bian C, Zhang D, Zeng Q, Xiang Z, Zhao A*. “Molecular cloning and expression analysis of mulberry MAPK gene family” Plant Physiol Biochem, 2014, 77: 108-116.
(31) Zhang Y, Long D, Zhang G, Bi L, Zhao A*. “Effect of transgene integration sites and copy numbers on hybrization and breeding in silkworm” Southwest China Journal of Agricultural Sciences, 2014, 27(2): 892-895. (in Chinese)
(32) Long D, Zhao A*, Xu Long, Lu W, Guo Q, Zhang Y, Xiang Z. “In vivo site-specific integration of transgene in silkworm via phiC31 integrase-mediated cassette exchange” Insect Biochemistry and Molecular Biology, 2013, 43(11): 997-1008.
(33) Liu X, Ding T, Wei C, Long D, Guo Q, Zu F, He N, Yu M, Xiang Z, Zhao A*. “An evaluation on salt and drought tolerance of F1 generations from 13 mulberry hybrid combinations” Science of Sericulture, 2014, 40(5): 764-773. (in Chinese)
(34) Zhao A*, Zhang Y, Long D. “Potential for genetic improvement of silkworm through molecular and transgenic approaches” CAB Reviews, 2013, 8(009): 1-9.
(35) Long D, Zhao A*, Chen X, Zhang Y, Lu W, Guo Q, Handler A, Xiang Z. “FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori” PLoS One, 2012, 7(6): e40150.
(36) Long D, Tan B, Zhao A*, Xu L, Xiang Z. “Progress in Cre/lox site-specific recombination system in higher eukaryotes” Hereditas, 2012, 34(2): 177-189. (in Chinese)
(37) Zhao A*, Long D, Ma S, Xu L, Zhang M, Dai F, Xia Q, Lu C, Xiang Z*. “Efficient strategies for changing the diapause character of silkworm eggs and for the germline transformation of diapause silkworm strains” Insect Science, 2012, 19: 172-182.
(38) Zhao A*, Long D, Tan B, Xu L, Xiang Z. “Progress in research of FLP/FRT site-specific recombination system in higher eukaryotes” China Agriculture Science, 2011, 44(15): 3252-3263. (in Chinese)
(39) Xu L, Zhao A*, Long D, Tan B, Xiang Z. “phiC31 integrase mediates genome manipulation in higher eukar-yotes” Science of Sericulture, 2011, 37: 507-519. (in Chinese)
（co-first author indicated by #, and corresponding authors indicated by *）
联系地址：重庆市北碚区天生路2号，西南大学（南区），
家蚕基因组生物学国家重点实验室，400716
电 话：023-68251903
E-mail：dplong@yeah.net